Name of instrument, m RealTime System composed of the msp and mrt modules. Country where designed/Manufactured/Reagents. The Abbott m System comprises the msp and mrz instruments, which are operated with separate System Control Center (SCC). J Clin Microbiol. Jun;47(6) doi: /JCM Epub Apr Performance of the Abbott real-time PCR assay using msp and .
|Published (Last):||23 May 2015|
|PDF File Size:||12.69 Mb|
|ePub File Size:||12.91 Mb|
|Price:||Free* [*Free Regsitration Required]|
The Abbott m RealTime System provides automation from bar-coded laboratory tube through patient result, creating an efficient workflow for your laboratory.
Review summary M Correa Posted on Dec 08 at 7: Home Publications Conferences Register Contact. Select your language of interest to view the total content in your interested language.
Detection by real-time technology used two probes of different lengths: Abbott Molecular’s msp for sample preparation and the mrt for real-time amplification and detection are the cornerstones for this process. Results of this study are unable to evaluate the potential differences between these two assays in this regard. Furthermore, the input plasma volume is different j2000sp the two methods. It is unclear at the moment the source of the discrepancies. Receive your quote directly from the manufacturer.
HIV viral particles were first lysed and an HIV-1 Quantitation Standard QS Armored RNA molecule was added to each sample, which was followed by a series of processes which involves incubation, introduction of magnetic abboott, washing to remove unbound particles and elution of nucleic acid NA at elevated temperatures.
In conclusion, the two methods showed strong correlation in measuring VL within their testing dynamic range. In this study, we have compared VL quantification by the Roche and the Abbott methods side-by-side. Thus some of the discordant samples could potentially be due to the HIV-1 subtype differences.
The fluorescence activity was converted into VL yields by the analyzer Roche, In addition, their principles in designing primers and probes are also different. In this configuration, signal is not created by probe hydrolysis but by separation of the probe from quencher oligonucleotide. October 18, Citation: The objective of this study was to compare results of HIV-1 VL quantification between m2000dp two methods.
Together, these data suggest that neither method is better than the other for measuring VL. This study was approved by the Institutional Research Board under the protocol number of H The fluorescence counts were converted into VL measure by the analyzer Abbott, a ; Abbott, Following reverse transcription, the RNA: Request Pricing Receive your quote directly from the manufacturer. This automation enables laboratorians to interface where their skills best contribute to results while minimizing errors and contamination.
Dual-labeled fluorescent probes for HIV-1 and HIV-1 QS-specific oligonucleotides were used, each labeled with different reporter dyes and a quencher dye.
Another common feature of these two assays is the use of realtime PCR as their underlying technology for the measurement of HIV- 1 viral load. It should be mentioned that the genetic diversity of HIV-1 subtypes could present a significant challenge in the accurate VL monitoring of HIV-infected patients who carry non-B subtypes Damond et al.
These two assays share three common features 1 Additional reduction in the lower limits of detection LOD of HIV-1 RNA from the earlier version; 2 New primers and probes designs for recognition of the different viral subtypes and circulating recombinant forms CRFs ; and 3 Reduction in hand-on time by configuring the test near to full automation. Table 1 summarizes the assay characteristics of these two assays.
mrealtime-system – hospicare
While the Roche method uses abboht standard TaqMan technology, a different and partially double stranded probe is introduced to the Abbott method Finan and Zhao, ; Johanson et al.
Pearson correlation test was used to measure the overall correlation of the VL between the two methods. Even though both the Roche and Abbott methods use the realtime PCR technology, the gene targets are different.
Different from the conventional PCR, abboyt internal probe agbott added to the detection process, which is an oligo nucleotide with both a fluorescent reporter and a fluorescent quencher dye attached.
The Abbott method positively identified samples out of with the detectability of abboht Out of paired plasma tested, Digestion of the probe DNA separates the reporter dye from the quencher dye, making the reporter dye signal detectable. All blood samples were collected in EDTA tubes. The solid line in the box indicates the median value and the ends of the whiskers indicate the minimum and maximum values. The extracted NA was mixed with the amplification reagents before amplification with the mrt Abbott, a ; Abbott, However, a relative high However, this factor should not be the major contributing factor because a recent surveillance study at the University of Maryland Medical Center showed that about The Abbott method uses real-time platforms with fluorescence detection systems Abbott, b ; Scott et al.
Our data showed that these two methods abboth essentially indistinguishable with a correlation coefficient of 0.
Abbott M2000sp Liquid Handler W/ M2000rt PCR
Consistently, comparison of the distribution and median values of VL abbitt these two methods also showed similar median VL values but a slight wider distribution by the Roche method than the Abbott method was seen Abbot 2.
Pharmaceutical Sciences Journals Ann Jose ankara escort. The number of samples within this range was Please leave a message, we will get zbbott you shortly. Real-time PCR was used for detection. The Version 2 of the m open mode 0.
In addition, different precision of the methods abobtt also potentially explain some of the discordant results. One way to further evaluate the cause of the observed discrepancies is to determine the HIV-1 subtypes by DNA sequencing. Guidelines Upcoming Special Issues.
Whether through new system software releases, innovative assay design or a constantly expanding mSystem menu, we are continuously evolving. Finally, the NA was released using an elution buffer. The difference between these two methods was Log10 0. Comparison of the mean VL with differences of the two methods using the Bland Altman plot showed rough symmetric distribution of the differences, indicating neither method is better than the other for measuring VL.