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Thus, gently removing the CS without touching the cervical surface should be recommended in any procedure for obtaining a cervical cytology sample. Ginecplogia, a higher rate of inflammatory reaction was found to be present in the CS compared to the SS, whereas more epithelial cells with clear nuclear details were present in the SS compared with the CS Figure 2likely owing to the fact that these are living cells that are attached to the cervical surface, and if these are actively removed, their cytological characteristics will be nobak well-preserved at the time of fixation Figure 3.

Support Center Support Center. P16 INK4a Immunohhistochemestry impreoves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. By excluding CS from cervical samples, the sensitivity could be improved and the false negative rate ginecologiz be reduced.

CS is the result of exfoliated cells from the cervix and vaginal wall, and contains inflammatory cells and bacteria in addition ginecologiq the mucus secreted from the endocervical glands, which are normally found in the cervical orifice.

The reference standard for the positive true diagnosis was the combined results of the biopsy and p16 staining, whereas a true negative diagnosis was based on negative ginecoloia for CIN in the biopsy [ 16 ] and negative p16 staining [ 1718 ]. Examples of different cervix, both with large ectopia, abundant mucus, with the brush, showing how to clean it getting the mucus to the top in A and left in B. In our study, colposcopy was used to detect small lesions, which were confirmed by cytology and diagnosed by biopsy.


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Clinical and Laboratory Standards Institute. Cervical mucus, liquid-based cytology, sampling studies, cervical cytology. Procedure for removing the cervical mucus.

Am J Surg Path. Ginecoolgia obtaining cervical samples, whether the samples are taken from the transformation zone and whether abnormal cells are missing must be considered.

La crétinisation par la culture

Evaluation of two simultaneously obtained cervical cytology smears. The age of the study cohort ranged from years, with an average age of 33 years. Cervicovaginal cytology based on the Papanicolaou technique: False positive frequency in CS samples. Schematic of the procedure for removing cervical mucus.

In this study, we analyzed samples obtained with or without cleaning the cervix, and compared their contents in order to determine the sensitivity and specificity of these two methods.

In this study, we compared the CS removed without touching the cervical surface to the SS to evaluate the adequacy of both samples, and to obtain and compare their sensitivity, specificity, and positive and negative predictive values. The brush is rolled gently without touching the cervical surface.

The SS resulted in a higher proportion of adequate samples being obtained The sensitivity and specificity were calculated using biopsy as the gold standard.

Sample characteristic and kind of inflammation detected in the histophatologycal analyze in CS and SS respectively. Obwegeser JH, Brack S. J Lower Gen Tract Dis. Am J Obstet Gynecol. Endocervical cytobrush with the cleaned-out mucus. Thieme Medical Publishers; If the CS is removed with an oversized or normal-sized cotton swab, it is likely that the swab touches the cervical surface and may result in some CIN cells being removed; and these would hence not be present in the clean, second sample SS.


This condition is rare and was detected from a thorough inspection of the vagina when sampling the cervix. Author information Article notes Copyright and License information Disclaimer.

The types of diagnosed CIN were divided as shown in Table 2. Novqk results of the present study raise an important question: An example of the amout of mucus retired.

The laboratory is a private practice, serving a low-risk population in Mexico City, Mexico. This could be justified in part by the presence of an atrophic cervix, a small TZ, or by the squamocolumnar junction not being visible.

The results are expressed in percentages and frequencies for all parameters. They found that after the cervix had been swabbed, more ASs were obtained but less CIN cases novai detected, and concluded that this was likely attributable to the age of the participants, as well as the possibility that some cases of CIN had cleared when the second study was being conducted.

National Center for Biotechnology InformationU. All biopsy samples were stained with hematoxylin and eosin as per standard protocol and analyzed by immunohistochemical staining for cyclin-dependent kinase inhibitor 2A p16 clone J6; Fe Marke, CA, USAusing the CIN classification [ 16 ]. The sensitivities for CS and SS were