Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.

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The AFLP system presents good levels of precision in its genetic estimates and single crosses prediction.

The past limitations associated with pedigree data and morphological, physiological and cytological markers for assessing genetic diversity in cultivated and wild plant species have tekknii been circumvented by the development of DNA markers such as restriction fragment length polymorphisms RFLPs; Botstein et al.

Theor Applied Genet The loci used were: Plant Breeding 6: I ; Anete P.

In this method, co-occurrences are divided by the total number of evaluated loci excluding the negative co-occurrences and thus can be interpreted aglp the proportion of coincidences in relation to the total number of evaluated loci. The coefficient of variation CV for all genetic distances across the bootstrap samples was estimated for each specific number of loci or bands sampled, a computer program for performing these analyses being set up using the ‘RANNUNI’ function of the SAS system Version 8.

Microsatellites SSRs occur frequently aglp most eukaryote genomes and can be very informative, multiallelic and reproducible Vos et al.

Materials and methods Alfp material and DNA isolation Eighteen S 3 selected inbred lines from two divergent tropical maize populations eight from BR and ten from BR previously had their genetic distances surveyed using four different marker systems Lanza et al. The RAPD markers were clearly the most distinct type of marker because the correlation values involving this marker were equal to or lower than 0.

Because the box-plots for each of our groups of samples were skewed we used the mean, median and maximum CV values to determine teinii adequate number of polymorphic loci needed for acceptable precision.

European Patent Application number: The objectives of the telnii described in this paper was: Ag Biotech News Inf 6: Each tekii visualized on the gel was considered to be the re-sampling unit for dominant markers because for these markers each band is related to one locus. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Bull Soc Vaud Nat Although similar average tekniu distance values were obtained for the BR and BR intrapopulation crosses, the BR crosses showed the widest range of genetic distances with all of the four different markers assayed; probably because of the broader genetic base of the BR population.


Bootstrap analysis were used to evaluate the number of loci for the markers and the coefficients of variation CV revealed a skewed distribution. Codominant markers relate each band to an allele, and therefore the boostrap was applied among locus.

Differences in the distribution profiles also occurred between dominant and codominant markers, with dominant markers having higher standard deviations than codominant markers. Comparisons with data from RFLPs and pedigree. To determine the sampling variance of the genetic distances produced by the different molecular data sets we performed bootstrap analysis using a decreasing number of loci tekini codominant markers or bands for dominant markers.

Barbosa I ; Isaias O. Results Levels of polymorphism All of the 18 maize inbred lines studied by us had previously been investigated using the four different marker systems RAPD: Jaccard similarities were calculated using version 2.

Although the discrimination power of RFLPs in diversity studies has been well documented Smith et al. From the analysis of our data it appears that the maximum CV value appears to be, in most cases, the best guarantee for producing reliable estimates of genetic qflp. We used the median and maximum coefficient of variation values to evaluate tteknii accuracy of the genetic distance estimates because although the mean coefficient of variation is often used in the literature caution is needed when dealing with molecular marker data for which there is no assurance tsknii the CVs values are distributed symmetrically.

For dominant markers, where the distribution is skewed towards lower genetic distance values, the use of mean or median CV values may lead to errors because some of the genetic distance values will not fall within the required level of precision.

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines

Bootstrap analysis was used to verify if the number of polymorphic loci evaluated was high enough to provide accurate genetic distance estimates King et al. Theor Appl Genet 94, Because the mean is not a good indicator of central tendency for skewed data we calculated the minimum number of loci necessary for an accurate representation of the genetic distances by fitting an exponential function based on the mean, median and atlp CV values of the genetic distances obtained by bootstrap sampling to the data for each marker, the results of this analysis being given in the Boxplots shown in Figure 3.


The results also suggest that the number of loci evaluated should be increased.

Due to this uniqueness, molecular markers have been very useful in genetic evaluations and assignment of tropical maize inbred lines to heterotic groups. Pearson’s correlation coefficient was calculated for the genetic distances, single cross performance and heterosis as previously described by Benchimol et al.

Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)

Calculations were made using the following formula: Correlations between genetic distances measured with different markers. J Genet Breed How to cite this article. Variability in Chinese sorghums. Comparison of pedigree and RFLP data. The RFLP system appears to be the most robust marker assay in terms of the amount of polymorphism surveyed, although, in practice, it is still a very laborious technique. Am J Hum Genet Our results indicate that, apart from the RAPD markers, the other DNA marker systems provided consistent information for diversity studies on tropical maize populations and produced genetic distance estimates which were in good agreement.

Nucl Acids Res All of the 18 maize inbred lines studied by us had previously been investigated using the four different marker systems RAPD: Garcia I ; Luciana L.

The results obtained in our study showed high agreement between RFLP and AFLP genetic distance estimates, such estimates having also been highly correlated in other studies Russell et al.

Correlation of genetic distance with F 1 grain yield and heterosis. However, in the case of maize, tropical and temperate populations differ from each other because tropical populations usually originate from composites with higher genetic variability and, most of the time, it is difficult to allocate tropical composites to well-defined heterotic groups by phenotypic evaluation.

Pesq Agropec Bras 28, Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands.